Pbac

Pbac serves as a specialized genomic resource for studying microbial communities in giant panda gastrointestinal systems. This curated database addresses the unique bamboo-digesting adaptations of endangered ursids through two major releases: ​Version 1​ established the foundational collection with 2,684 metagenome-assembled genomes (MAGs) derived from hybrid Illumina-Nanopore sequencing, including 354 high-quality representatives that revealed significant novel microbial diversity. Version 2​ substantially enhanced genomic quality through integrated culturomics and PacBio HiFi sequencing, expanding to 466 species-level references (84% increase) with 213 newly documented species and five novel genera. Validation shows >92% mapping efficiency for microbiome analyses. Researchers can leverage Pbac for diverse applications including metagenomic profiling, conservation genomics, and investigating host-microbe coevolution in bamboo-digesting ecosystems. The database provides both individual genome access and bulk downloads of pre-compiled Kraken2 databases for efficient analysis pipelines.

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Data Resource

Provided resource in Pbac v2 database.

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Recent News & Blogs

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Long-Read Metagenomic Sequencing of a Pooled Fecal Sample from Giant Pandas (ONT & PacBio HiFi)

A pooled fecal sample from three giant pandas was sequenced using Oxford Nanopore Technologies (ONT) and PacBio HiFi platforms, and the datasets......

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03

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Analysis of Giant Panda Gut Microbial Metagenomic Data Using Kraken2 and PBac v2 Databases

We have pre-built Kraken2 (v2.1.2) and Bracken (v2.9) reference database packages based on the PBac v2 database, which can be downloaded from this......

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Taxonomic Relative Abundance Estimation via Kraken2 and Bracken

1. MAGs Generation Workflow2. Isolated Bacterial Genome Assembly Workflow3. Taxonomic Relative Abundance Estimation via Kraken2 and Bracken4.......