NEWS & BLOGS
Long-Read Metagenomic Sequencing of a Pooled Fecal Sample from Giant Pandas (ONT & PacBio HiFi)
Published on: 2025-09-04 | By: EDITOR
A pooled fecal sample from three giant pandas was sequenced using Oxford Nanopore Technologies (ONT) and PacBio HiFi platforms, and the datasets have been deposited in public repositories; see the table below for details.
| Sample | Clean of ONT Reads (Nanopore) | Clean of HiFi Reads (Pacbio) | ||||||||
| Num. clean reads | N50 (bp) | N90 (bp) | Longest (bp) | Total Base (Gb) | Num. clean reads | N50 (bp) | N90 (bp) | Longest (bp) | Total Base (Gb) | |
| DD | 5,555,826 | 11,578 | 7,425 | 197,796 | 60.82 | 3,142,560 | 8,911 | 6,118 | 45,557 | 27.03 |
| DG | 4,280,192 | 18,200 | 8,144 | 182,734 | 59.84 | 3,529,069 | 8,175 | 6,103 | 43,288 | 28.47 |
| DJ | 4,831,729 | 15,588 | 8,522 | 195,272 | 63.49 | 1,780,029 | 4,007 | 1,624 | 30,025 | 5.62 |
The raw sequencing data are available for download from NCBI: https://www.ncbi.nlm.nih.gov/sra?linkname=bioproject_sra_all&from_uid=1073587
Please cite the following article:
(3) Deng, F., Han, Y., Li, M., et al. (2025). HiFi based metagenomic assembly strategy provides accuracy near isolated genome resolution in MAG assembly. iMetaOmics 2 (3), e70041. https://doi.org/10.1002/imo2.70041